Acid-Base Titration
Acid/base titrations can be used to determine the buffering capability of a particular amino acid. Acid or base is added dropwise to a solution containing the amino acid of interest and to a solution containing only water. Because of the buffering ability of the amino acid, the solution with the amino acid will resist a change in pH around the pKa values of its functional groups. The solution with water is a control to compare what the pH would be without the buffer. The behavior of each solution in response to the addition of more acid or base can be analyzed with a titration curve.
